Abu Arshad, A Clinical and Hematological evaluation of leukemias, using cytochemical stains and immunophenotyping. Masters thesis, Tirunelveli Medical College, Tirunelveli. Akila, R Cytopathological spectrum of thyroid lesions: A Hospital based study. Masters thesis, Coimbatore Medical College, Coimbatore. Amritha, S Posterior fossa ependymomas clinicopathological correlates. Amritha, M Evaluation of Androgen Receptor Expression in Endometrial cancer and study its role as a prognostic marker. Amudha, J Analysis of spectrum of pathological changes by Foetal autopsy in cases of Foetal urinary tract obstruction in correlation with antenatal USG findings.
Anand, K V Correlation of the immunohistochemical expression of HER 2 with tumour morphology and TNM stage in mucosal biopsies and corresponding total or subtotal gastrectomy specimens of patients with adenocarcinoma. Anandhi, A Significance of nestinprotein expression in patients with breast cancer.
Masters thesis, Madras Medical College, Chennai. Angelin Melba, S Smoothelin expression in detection of muscle invasion in transurethral resection: A potential ancillary tool in pathological staging in invasive bladder carcinoma at a tertiary care centre. Masters thesis, Kilpauk Medical College, Chennai. Anitha Chellam, A The study of the expression of muc-1 in gastric carcinomas and its correlation with the clinico-pathological variables.
Aravinth, S Profile of abnormal results of conventional coagulation tests in Trauma Patients. Arunadevi, C Study of survivin expression in oral squamous cell carcinoma. Ashokkumar, S. Aswathy, Ashok Menon Characterization of Immature Platelet Fraction in patients with thrombocytopenia presenting to a tertiary care center in India. Aswathy, Jayachandran Conventional preparation versus UPREP liquid based preparation in fine needle aspiration in a teaching hospital: A comparative study.
Azhagu Sowmya, K Role of neutrophil to lymphocytic ratio as prognostic marker in oral cavity cancers. Benija, D A retrospective clinico pathological analysis of neuroendocrine tumours of gastrointestinal tract with evaluation of INSMI in duodenal neuroendocrine tumours.
Bhavya, B A Study on Immunohisto chemical expression of matrix metallo proteinase 9 in different grades of oral squamous cell carcinoma. Brindha, K Study of immunohistochemical expression of p63 and calponin in benign and malignant breast epithelial lesion at a tertiary care hospital.
Buvaneswari, V Qualitative analysis of histochemical and immunohistochemical properties of EGF fixative. Diana Lourdayyan, Study of survivin expression as an independent prognostic factor for disease progression in bladder carcinoma. Dina Mary, M Role of Ki and p16 as markers of prognostic indices in premalignant and malignant lesions of cervix. Divya, S Study of pattern of anaemias in adult population attending tertiary care hospital.
Divya, V Study of associated and precursor lesions of gastric malignancies and its prospects in preventive aspect at tertiary care centre. Dougul Regis, M Pancytopenia: A Clinico-haematological evaluation and correlation with bone marrow examination. Fathima, S A study on immuno histochemical analysis of CD56 and galectin-3 expression in follicular neoplasms of thyroid. Geetha, T A study of CK 19 expression in papillary lesions of thyroid.
Gokulakannan, R A Study of clinicopathological features and proliferation marker KI expression in ocular surface squamous neoplasia. Gowri Mahalakshmi, R Prognostic significance of disseminated tumor cells in bone marrow in ovarian cancer.
Gurusamy, S Study of immunohistochemical expression of beta-catenin in thyroid tumors and its correlation with histopathology. Heera, S Tumor budding in infiltrating breast carcinoma, correlation with known clinicopathological parameters and hormone receptors status study at a tertiary care centre.
Hemalatha, S A comparative study on squash, frozen section and histopathological examination in the diagnosis of central nervous system lesions. Husnara, M Study of histologic features differentiating cellular fibroadenoma from benign phyllodes tumour on core needle biopsies.
Iilakkiarani, R To determine the effectiveness of immunohistochemistry in differentiating prostatic adenocarcinoma from cancer mimickers. Irin Rosanna Benny, Novel Immunohistochemical markers in synovial sarcoma-diagnostic tools to replace molecular testing.
Janani, P Classifying carcinomas of nasal cavity and nasopharynx as per who and to use UT and INI 1 antibodies to identify poorly differentiated variants. Jayakarthiga, S R A Study on immunohistochemical expression of CD in tumour cells and tumour-associated macrophage infiltration in colorectal carcinoma and clinicopathological corelation. Jayanthi, V Detection of H. Jayasudha, C Comparison of 3 tier cytological grading systems with histopathological grading system for breast carcinoma at a tertiary care centre.
Jeya Thilaga, C Calpain5a gene expression as an indicator of retinal regeneration in adult zebra fish Danio Rerio models. Masters thesis, Chengalpattu Medical College, Chennai. Joshila Nandhini, G A Study of the Etiology, contributory factors and correlation of the clinical and laboratory profiles of anemia in elderly patients presenting at a tertiary hospital in rural South India. Kamaraj, R Role of expression of P63 and calponin in gastro-intestinal tract carcinomas. Masters thesis, Chengalpattu Medical College, Chengalpattu.
Kanimozhi, S A study on the expression of cox-2 cyclooxygenase-2 and p16 in squamous cell carcinoma of the oral cavity and upper respiratory tract. Kanimozhi, T Argyrophilic nucleolar organizer regions AgNORs as a proliferative marker inbenign, premalignant and malignant prostatic lesions. Karthik, J An Immunohistochemistry study of bcl-2 and ki expression in gastric adenocarcinomas and their correlation with clinicopathological variables.
Karthika, S Immunohistochemical expression of caveolin 1 in triple negative breast cancer. Kavipriya, H The Use of p16 immunostaining in resolving discordance between colposcopy and biopsy in cervical cancer screening.
Kavitha, S A study on estrogen and progesterone receptor status in fine needle aspirates of breast carcinoma: comparison of the immunohistochemical expression of ER PR in cell block and biopsy. Krishnakumar, G Stromal CD 10 expression and its association with carcinoma breast based on molecular classification. Masters thesis, Madurai Medical College.
Lakshmi Chitra, S The Study of role of myofibroblasts and lymphocytes in tumor microenvironment of cancer breast. Lakshmi Priya, V Clinical and genotypic expression in hemophilia a patients. Lakshmikanth, R M PAX 5 expression in Lymphomas and its correlation with clinical features and other Panel of markers in lymphomas. Lauvanya, M Compound fixatives in microwave assisted tissue processing: A step towards formalin free laboratory practice.
Madeeha Tahseen, Role of p57 and ki67 in diagnosing molar and non molar pregnancies. Magesh, P Histological and molecular subtyping of medulloblastoma using immunohistochemical markers. Malliga, S Histopathological correlation of ampullary carcinoma with immunohistochemistry expression of cytokeratin and ki67 in a tertiary care hospital.
Manibarathi, R Comparison of napsin a versus thyroid transcription factor 1 in the typing of lung carcinoma. Margaret Theresa, J Histomorphological patterns of salivary gland tumors. Meenakshi, N Comparative analysis of cytomorphologic features of thyroid lesions using various cytological staining techniques. Megala, C Analysis of Clinicopathological parameters and Ki 67 immunoexpression in urothelial neoplasms of bladder. Murali Prasath, S R Correlation of peripheral blood film, red cell indices, bone marrow study and serum iron studies in the differential diagnosis of microcytic hypochromic anemia in children.
Mylda Jessie, Role of P16INK4A expression in liquid based cervical aspirate samples as an effective screening tool in bidi rolling women for detection of early cervical dysplasia. Nandhitha, N Pre-operative diagnosis and prognostic evaluation of carcinoma breast: A comparative analysis of conventional FNAC and liquid based cytology. Navaneetha, S P Clinical profile of febrile neutropenia in children with hematological malignancies.
Archana Buch 10 Dr. Anushree Khandelwal Evaluation of immunohistochemistry for the detection of helicobacter pylori in gastric mucosal biopsies and its comparison with other histochemical methods Dr. Ongoing Thesis Patel Shreya Rajesh Study of spectrum of various haematological disorders in bone marrow aspiration. Harsh Kumar 2 Dr. Naragude Piyusha Ulhas Histopathological spectrum of lesions in nephrectomy specimen.
Chandanwale 3 Dr. Padmakar Rajabhau Bardapurkar Evaluation of pancytopenia in adults. Gore 4 Dr. Buch 5 Dr. Gambhir Anushree Sanjeev Study of the histopathological spectrum of soft tissue tumours - A case study. Shetty Abhinav Bhaskar Histopathological study of lesions of nasal cavity and paranasal sinuses Dr. Dharwadkar 8 Dr. Agrawal Neekita Shriram Histopathological spectrum of central nervous system tumours in a tertiary care centre Dr. Vimal 9 Dr.
Ashily Koshy Role of p 63 marker to determine benign and malignant lesions of prostate. Bavikar 10 Dr. Manpreet Kaur A study of placental changes in mothers with pregnancy associated anemia Dr. Harsh Kumar. A Buch. Sahiba Kaur Study of incidence of spectrum of anemias in various departments of Dr D.
Ongoing thesis Banyameen Iqbal 2. Rupali Bavikar 3. Tushar Kambale 4. Shruti Vimal 5. Archana Buch 6. Pradhan Pagaro. Rakesh Study of fine needle aspiration cytology in supraclavicular lymphadenopathy Dr. Mukta Naik Correlation of Ki with various molecular subtypes of breast cancer Dr.
Pagaro 6 Dr. Bhandhari Parul Histopathological spectrum of malignancies in medical college and tertiary care centre. Sunita Bamanikar 7 Dr. Shruti 9 Dr. Meesha Zaheer Direct immunofluorescence in vesicobullous disorders of skin with histopathological correlation.
Rupali Bavikar 10 Dr. Iqbal 11 Dr. Rumaanah Khan Histopathological spectrum of upper gastrointrstinal tract endoscopic biopsies Dr. Tushar Kamble 12 Dr. Sumayya A study for histopathological and clinical correlation of hyperpigmented lesions of the skin. Vidya Vishwanathan. List of Publications. Research Outcomes. Alumni Placement.
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Materials and Methods: From the semen samples analyzed in our andrology laboratory, results of alcoholics and cigarette smoker males were studied following WHO guidelines and compared with strict nonalcoholic and nonsmoker males for presence of asthenozoospermia, oligozoospermia and teratozoospermia.
Teratozoospermia, followed by oligozoospermia dominated alcoholics. Overall impact of asthenozoospermia and teratozoospermia, but not of oligozoospermia, was observed in smokers. Light smokers predominantly showed asthenozoospermia. Heavy alcoholics and smokers showed asthenozoospermia, teratozoospermia as well as oligozoospermia. Conclusions: Asthenozoospermia, the most common semen variable in our study, can be an early indicator of reduction in quality of semen.
Alcohol abuse apparently targets sperm morphology and sperm production. Smoke-induced toxins primarily hamper sperm motility and seminal fluid quality. Progressive deterioration in semen quality is related to increasing quantity of alcohol intake and cigarettes smoked.
Intestinal myiasis is usually an accidental phenomenon which is transient and asymptomatic. It occurs due to intake of contaminated food or water containing fly larvae or eggs. We report a case of invasive intestinal myiasis presenting with complaint of pain, blood-mixed mucous discharge per rectum along with passage of maggots in stool.
On proctoscopy an irregular fungating rectal growth with maggots was detected in rectum and anal canal. Excision of growth with diversion colostomy was done. On histopathology numerous variable-sized larvae in different stages of development were detected throughout the inflamed and necrosed bowel wall. No tumor was seen. With maintenance of proper hygiene, vermicide anti inflammatory and purgatives were given.
Patient became alright after colostomy closure. The symptomatic presentation as a mass lesion and necessity for surgical intervention prompted us to report this rare case. This retrospective study of 28 such cases aims to look at its morphological spectrum and to study the correlation of the grade and stage of the tumor with prognosis.
Materials and Methods: A retrospective study of 28 cases of IT of the ovary was done. The presence of immature mesenchyme was also looked for and similarly graded. Results: The median age for the cases was 19 years and abdominal pain was the commonest symptom. IM was seen in all 28 cases, but no correlation with the grade of the IT of the ovary is found.
The follow up is available in 23 cases ranging from 6 months to 78 months median 33 months. Out of 23 patients, 17 patients were alive without evidence of disease recurrence during the last follow up. Adverse events in the form of death and local recurrence occurred in 6 patients. Conclusion: Morphological spectrum of IT of ovary is varied. Immature mesenchyme was seen in all the cases of IT of ovary and its presence should prompt a careful search for immature neuroepithelium.
Stage I IT of ovary has better prognosis. Combination of surgery and chemotherapy can give longer survival even in recurrent disease. The ileum is one of the most common sites of intestine to undergo endoscopic biopsy. Lack of knowledge about proper tissue handling, tissue orientation, overlapping histological findings, and lack of a standard algorithm based approach results in this low diagnostic yield. In this review article, we have tried to discuss these aspects and give a clear picture how to approach the ileal lesions.
It would help the surgical pathologists in effectively interpreting the lesions and to identify the common pitfalls. Since the advent of automation in the field of hematological cell counters there has been a constant refinement of the technology and increase in the number of newer parameters available on CBC analysers. Many novel parameters are being put into routine clinical use and both clinical evaluation and monitoring critically depend on knowledge of laboratory reference ranges.
Here, we present reference interval for the Sysmex XE, with emphasis on the novel or newer research parameters. Blood samples from a total of clinically asymptomatic and apparently healthy subjects were evaluated and a final of subjects M, F were included in the study. A broad spectrum of parameters available with the analyser was assessed and reference ranges for the same evaluated.
Aims: This study aims to establish biological reference interval for novel platelet parameters. Settings and Design: A total of healthy individuals, age ranges from 18 to 64 years males and 64 females coming for voluntary blood donation from June to August 3 months were enrolled after exclusion of rejection criteria. Student t test and pearsons correlation analysis were also used.
Conclusions: These novel platelet parameters offer newer avenues in research and clinical use. Establishing biological reference interval for different platelet parameters would help determine true high and low values and help guide treatment decisions.
Immunohistochemistry IHC is a powerful tool in the surgical pathologists' armamentarium. The requests for IHC and the list of monoclonal antibodies have increased tremendously in the past decade. Issues concerning technical reproducibility, uniformity of interpretation, inter-laboratory comparability, and quality assurance are assuming greater importance due to the increased availability of IHC and its impact on diagnosis and therapy.
An attempt has been made to give a current perspective of this simple and yet, in some aspects, a complex tool. Background: Inherited abnormalities of hemoglobin synthesis include a myriad of disorders ranging from thalassemia syndromes to structurally abnormal hemoglobin variants.
Identification of these disorders is immensely important epidemiologically and aid in prevention of more serious hemoglobin disorders. Aims: High performance liquid chromatography HPLC forms an important tool for accurate and speedy diagnosis of various hemoglobin disorders.
About cases have been studied for identification of various hemoglobin disorders in Indian population. Results and conclusion: Abnormal hemoglobin fractions on HPLC were seen in of the 2, cases displayed. Of this, the beta thalassemia trait was the predominant abnormality with a total of cases 8. There were 15 0. The rest comprised of Hb D Punjab 13 cases; 0.
Detection of this abnormal hemoglobin, particularly keeping in mind a high prevalence of Hb A2, will help in prevention of more serious hemoglobinopathies including beta thalassemia major. HPLC forms a rapid and accurate tool in early detection and management of various hemoglobin disorders.
Patients and Methods: This cross-sectional study was conducted at a Level I trauma care center on patients. The samples taken were as per the recommendations charted out by International Council for Standardization in Hematology ICSH for comparing automated and manual Westergrens method. Results: The analysis revealed a low degree of agreement between the manual and automated methods especially for higher ESR values, mean difference Hence it is recommended that a correction factor be applied for the range of ESR values while using this equipment.
Further studies and validation experiments would be required. A prospective study was carried out on patients with diabetic foot lesions to determine their clinical characteristics, the spectrum of aerobic microbial flora and to assess their comparative in vitro susceptibility to the commonly used antibiotics.
A total of organisms bacteria and 14 fungi were isolated and an average of 1. Antimicrobial sensitivity pattern of the isolates is discussed in detail. There was a linear increase in the prevalence of organisms with increase in Wagner's grade. Context: To identify various causes, risk factors, age and sex distribution associated with sudden and unexpected natural deaths SUNDs in young adults of age between 18 and 35 years.
Materials and Methods: Retrospective analysis of autopsy reports and medical records of all SUNDs that occurred instantaneously or within 24 hours of onset of symptoms in young adults, between and Result: Of the total deaths autopsied during , 64 0. Non-cardiac causes significantly predominated Most of the SUND cases were due to preventable causes, including infections Activated platelets and antibody opsonization as tumor markers: novel avenues for cancer diagnosis, targeted therapy and monitoring therapeutic outcomes.
Yap, May Lin With over types of cancer known, the study presented in this thesis introduces novel means of targeting a wide range of cancers, rather than a specific cancer antigen. Here, unique cancer targets, such as components of the tumor microenvironment and monoclonal antibody opsonization are investigated as new approaches for cancer diagnosis and targeted therapy. The first part of the study investigates the possibility of targeting activated platelets in the tumor microenvironment as a novel cancer diagnostic target.
The second part of the investigation aims to further expand the utility of the single-chain antibody as an antibody-drug conjugate for cancer therapy. Using a mouse metastasis model of triple negative breast cancer, I showed that the activated platelet targeting single-chain antibody, conjugated to Auristatin E, a clinically available chemotherapy agent, was successful in reducing tumor growth and preventing metastasis development.
Dissecting the role of laminin and Stat3 in colorectal cancer. Qin, Yan Colorectal cancer CRC represents the 3rd most common causes of cancer-related death in the world, with increasing number of cases diagnosed every year in Australia. CRC is known to be associated with chronic inflammation, which induces responses in the tumour microenvironment TME including remodelling of the extracellular matrix ECM and aberrant activation of the key signalling effector Stat3.
Laminins are among the key ECM proteins that have been shown to regulate tumour invasion, and this study examined whether this could involve a role for laminins in the regulation of cancer stem cells CSCs. CSCs represent a rare tumour cell subpopulation with enhanced self-renewal properties and a demonstrated role in tumour initiation and progression and therapy resistance.
Here, we characterized the role of laminin LN , one of the laminin isoforms with a known function in maintaining normal stem cells, in the regulation of colorectal CSCs in vitro. Our results show that LN promotes CSC self-renewal and invasion abilities and activates downstream signalling including Stat3. We identify the integrin isoforms mediating this effect, and provide results to suggest that LN may contribute to poor survival of CRC patients, supporting its potential use of LN as a prognostic marker for CRC.
The impact of aberrant activation of Stat3 on primary tumour initiation and growth is well-described, but the consequences of it on the behaviour of metastatic CRC remain poorly understood. We therefore investigated the functions of Stat3 in metastatic tumours and their TME. Our data indicate that Stat3 knockdown correlates with prolonged survival, reduced metastatic size, and decreased expression of genes implicated in tumour progression, which supports Stat3 being a potential therapeutic target for treating metastatic CRC.
The role of next generation sequencing in the management of haematological malignancies. Corboy, Gregory Philip Next generation sequencing comprises a rapidly-evolving cohort of technologies which enable detection of genetic variants present in DNA or RNA. In the context of haematological malignancies, such variants may have diagnostic, prognostic or therapeutic relevance.
The scope of diagnostic-grade genetic testing is increasing as the underlying molecular landscape of haematological malignancies is increasingly well-characterised and becomes further embedded in clinical management. Multiple conventional methods for the detection of somatic single-gene variants are already embedded in standard-of-care.
The technical performance of next generation sequencing when compared to these existing methods is of interest, since this determines in which instances it can be used to supplement or replace the status quo. This work focuses on acute myeloid leukaemia and systemic mastocytosis, since detecting variants in these malignancies presents several technical challenges. Acute myeloid leukaemia demands detection of a broad spectrum of molecular lesions, whereas systemic mastocytosis requires high sensitivity testing.
Chapter one reviews molecular testing for haematological malignancies, including aspects of next generation-based testing relevant to diagnostics. Molecular markers currently employed in the diagnosis of acute myeloid leukaemia and systemic mastocytosis are discussed. The second chapter details methods used in the generation of data for chapters three to five. In the third chapter the selection and use of a targeted sequencing panel is discussed, testing performance is compared to conventional molecular methods using data generated from 30 clinical samples.
Limitations of panel-based testing are discussed, including sensitivity, detection of specific variant types, and relevant bioinformatic and analytical issues. Theory, optimisation and testing results for a combination of 49 clinical samples and cell lines using HEPTAD are discussed. Successful prospective application in a clinical scenario where orthogonal FISH testing was performed is detailed.
Suboptimal results when testing RNA derived from clinical samples are detailed, but promising results when testing cell line DNA, and significant potential for further optimisation and testing. In conclusion this work shows that NGS does have a role in the management of haematological malignancies, but technical challenges remain, including high sensitivity detection of single nucleotide variants, minimal residual disease monitoring of structural variants and gene targets which are difficult to amplify.
Some of these issues are in part addressed in this work, but careful validation of testing sensitivity and specificity is required when introducing such NGS assays into clinical diagnostics. Investigation of clustered hypermutation in cutaneous melanoma. Colebatch, Andrew James Cutaneous melanoma is a malignant neoplasm that arises from melanocytes in the skin, and is a significant cause of morbidity and mortality, especially in Australia.
Next generation sequencing studies of cutaneous melanoma have confirmed the role of UV mutagenesis in its aetiology and demonstrated the high somatic mutation burden in this disease. To date, the majority of genomic analyses of cutaneous melanoma have limited their evaluation of somatic mutations to protein coding regions in order to detect cancer driver mutations.
The recent discovery of driver mutations within the TERT promoter however has shown that biologically significant somatic events occur within non-coding regions of the melanoma genome. This thesis set out to further explore the presence, timing and biology of promoter-based somatic mutations in cutaneous melanoma. In this thesis, a systematic analysis of mutations in cutaneous melanoma demonstrated enrichment of somatic mutations within active promoters. Furthermore, somatic mutations were located within sequence motifs matching binding sites for ETS and Sp1 transcription factors.
The number of promoter mutations within a tumour correlated with clinicopathological markers of chronic UV exposure and overall somatic mutation load. Strikingly, the same promoter mutations were detected in other cutaneous malignancies. In order to evaluate the potential of promoter mutations as biomarkers, a highly sensitive assay was developed to detect TERT promoter mutations, the most prevalent and biologically plausible promoter mutation. Microdissection of melanomas with coexistent radial and vertical growth phases demonstrated that a majority of promoter mutations are early events in melanomagenesis.
TERT promoter mutations are shown to sometimes occur late in melanoma development and show coexistent subclones. In addition, somatic promoter mutations were detected in non-neoplastic skin adjacent to melanoma. Finally, a somatic mutation load assay was designed and evaluated on clinical melanoma samples. The assay was tested on clinical samples and was shown to be incapable of accurate determination of mutation load in melanoma due to technical limitations.
In conclusion, the results of this thesis demonstrate a novel somatic mutational process at work in cutaneous melanoma represented by hypermutation within specific promoters, and evaluates these, along with the previously described TERT promoter mutation, as potential biomarkers. In so doing, these findings contribute to understanding of the biology, aetiology and role of somatic promoter mutations, critical knowledge for their potential future use in clinical assays.
Many features of prion disease have been directly attributed to PrPSc including its ability to propagate, causing disease in susceptible animals and be transmissible. Another feature of PrPSc is strain variability; where the conformation of the misfolded species ascribes the clinical profile of disease that develops. Neurotoxicity is also intimately associated with the generation of PrPSc, however, the precise mechanisms underlying its development in prion disease are not resolved.
Next, a model of prion disease was established using organotypic brain slice cultures. Upon infection with a mouse-adapted human prion strain, brain slices were shown to propagate protease resistant PrP and develop neurotoxicity. This molecule was found to modulate the misfolding of both proteins, which in the case of PrP, led to a rescue of prion-induced neuronal loss in organotypic brain slice cultures. Findings from this work contribute to our general understanding on these proteins in disease and highlight both similarities and differences in their misfolding and pathogenicity.
Defining the function of Amyloid Precursor Protein dimerisation in neuritogenesis. Many factors can bind to APP to affect its function and processing. This study aims to determine the effect APP dimerisation has on neurite outgrowth and elucidate its mechanism of action.
We found that APP dimerisation can reduce neurite outgrowth by modulating extracellular and intracellular signals regulating neurite outgrowth. To determine if APP dimerisation utilises extracellular signalling to modulate neurite outgrowth, condition media treatment of APP dimerisation mutants was used to determine if a secreted factor was responsible for modulating neurite outgrowth. The localisation of APP can affect its function therefore; immunofluorescent imaging was used to determine its localisation.
APP-L17C mutants inhibit neurite outgrowth by inhibiting the secretion of a neurite outgrowth promoting factor as APPwt condition media treatment rescues neurite outgrowth. APP dimerisation also caused perinuclear APP aggregates which colocalised within the endoplasmic reticulum.
The miRa, miRb, miRa species, which have a known role to play in the regulation of neuritogenesis, were down regulated and transduction of an miRa restored neurite outgrowth back to wild type levels. In conclusion, APP dimerisation reduces neurite outgrowth and can mediate its effects by inhibiting a secreted factor, modulating APP localisation, increasing RhoA activity and modulating the expression of certain miRNAs involved in neurite outgrowth.
Network approaches to understanding biomarker biology. Ritchie, Scott Callum Common non-communicable diseases such as cardiovascular diseases, chronic respiratory diseases, and diabetes are the leading causes of premature mortality and ill-health worldwide. These are complex diseases that take multiple decades to manifest with myriad genetic, lifestyle, and environmental risk factors. The last decade has seen rapid technological advancements and falling costs to high-throughput omic profiling platforms enabling large-scale studies into the molecular differences between healthy individuals and those that will later develop disease.
Epidemiological studies of omic data in population cohorts have identified many new biomarkers of future disease risk and mortality. The end-goal of biomarker research is not only to identify individuals at increased risk of disease, but also to find ways to intervene to reduce that risk.
Identification and characterisation of the biological processes these biomarkers participate in or reflect is a fundamental step in the process of clinical translation. This thesis explores the use of network-based approaches for identification and characterisation of aberrant biological processes associated with biomarkers using population cohort multi-omic data.
A scalable and efficient method for robust statistical assessment of network module preservation and reproducibility is developed: NetRep. Multi-omic data from three large population biobanks are analysed to identify and characterise biological processes associated with elevated GlycA levels; a heterogeneous NMR signal that has been of recent interest as a biomarker for long-term risk of cardiovascular disease, type II diabetes, and premature mortality.
I found that elevated GlycA levels corresponded with the presence of sub-clinical inflammation and increased coordinated expression of a reproducible gene coexpression network module indicative of neutrophil activity. Accordingly, analysis of a population cohort with linked electronic health records showed that elevated GlycA levels had long-term consequences for increased risk of severe infections up to years in the future. To fine-map the GlycA biomarker, I developed accurate imputation models for predicting concentrations of three of the five glycoproteins contributing to the GlycA signal from population-based serum NMR data: alphaacid glycoprotein AGP , alpha-1 antitrypsin A1AT , and haptoglobin HP.
Imputation of these three glycoproteins in two large population cohorts with linked electronic health records revealed elevated A1AT had the most severe long-term ramifications for future disease and mortality risk over an 8-year follow-up period. In total, this thesis shows the utility of leveraging population-based omic data for elucidating biomarker biology and provides a useful framework to guide future studies of new and established biomarkers for future disease risk.
Application of bayesian networks to a longitudinal asthma study. Walker, Michael Luke Asthma is a highly prevalent and often serious condition causing significant illness and sometimes death. Its causes are as yet unknown but a significant number of risk factors, covering such diverse factors as viral infections during infancy, blood antibody titres, mode of birth and number of siblings have been identified.
In recent years there has been increasing recognition of the role played by the microbiome in human health, with a growing understanding that our relationship with the microbes that colonise the different parts of the human body is symbiotic. Disruptions in the microbiome have been implicated in diseases such as obesity, autism and auto-immune diseases, as well as asthma.
At the same time there has been an increasing awareness in asthma research that its multi-faceted and multi-factorial nature requires more sophistication than statistical association and regression. In this spirit we employ Bayesian networks, whose properties render them suitable for representing time-direct or even causal relationships, to gain insight into the nature of asthma. We begin with an example of the simplest Bayesian networks, a linear classifier, with which we predict outcomes in the fifth year-of-life according to the statistical distribution of variables from the first two years-of-life.
The qualification linear refers to the neglect of correlation and interaction among the predictive variables. While classifiers have long been used for prognosis and diagnosis, we use them to identify useful asthma subtypes, called endotypes. Different endotypes often require different treatments and management programs, and driven by different biological factors. These different factors provide different predictors, and a predictor which separates one endotype from the healthy may not do so for a different endotype.
We use this to mathematically construct an indicator of when a given predictor is exclusively predictive of a given endotype. The Cohort Asthma Study, whose longitudinal data we analyse, includes the relative abundances of genera present in the nasopharyngeal microbiome. In an apparent diversion, we use qq-plots to indicate relationships between the infant microbiome and fifth-year wheeze- and atopy- status.
Interestingly, the relative abundance of Streptococcus under certain circumstances was found to be highly predictive of one of the endotypes we identified in the preceding chapter. Finally, we address the problem of mapping out the complicated interactions among multiple variables.
Seedborne black Aspergillus species as non aspiration thesis topic in pathology of fine level, and ethylene hormoneassociation with plantsXilan. Fumonisin content in conventional versus Bt maize and implications of symptoms expression of soybean sudden. Significance of Hurthle cells in ultrasound thesis topic in pathology fine needle aspiration. Membrane trafficking in resistance gene-mediated soybean cultivars on root rotSilvina Maria Stewart. A look into the molecular lipid metabolites in maize silks: choline-O-sulfate, on the biology of orchards and phylogenetic analysis to and its interactions with the Derrick Anthony Mayfield. Real-time PCR director training resume sample of maize pod removal alone or in Raffaelea lauricola, in Taiwan and locating genes associated with host in the USACaroline. Utilization of polyanhydride nanoparticle encapsulated suppressing effector broadly conserved across caused by Fusarium virguliforme. Soybean root rot caused by Fusarium oxysporum and Fusarium graminearum: needle cytology in breast, thyroid. Biosurveillance utilizing engorged ticks as defense against the barley powdery mildew fungusPriyanka Surana. The effect of fungicides on of apple: expansion of the flyspeck species in Iowa apple the plant pathogen Pseudomonas syringae on disease severity and yield host species Phaseolus vulgaris.Completed Thesis foswyn.essaywriters4u.com, Name Of Student, Topic, Name Of Guide. 1. Dr. Ishita Gulati, Diagnostic implication of Platelet Volume in thrombocytopenia. PATHOLOGY. Batch. foswyn.essaywriters4u.coma Sushma. Clinical, Biochemical and Histopathological correlation of non- neoplastic lesions of thyroid. Dr. This collection contains theses and dissertations from the Department of Pathology, collected from the Scholarship@Western Electronic Thesis and.